Pulsed Field Electrophoresis of Genomic Restrietion Fragments for the Detection of Nosocomial Legionella pneumophila in Hospital Water Supplies
نویسندگان
چکیده
Pulsed Field Electrophoresis of Genomic Restrietion Fragments for the Detection of Nosocomial Legionella pneumophila in Hospital Water Supplies MAN:FRED OTT,1 LARISA BENDER,1 REINHARD MARRE,2 AND JÖRG HACKER~* Institut für Genetik und Mikrobiologie, University of Würzburg, Röntgenring 11, W-8700 Würzburg, 1 and Institut für Medizinische Mikrobiologie, Medical University at Lübeck, W-2400 Lübeck, 2 Germany
منابع مشابه
Pulsed field electrophoresis of genomic restriction fragments for the detection of nosocomial Legionella pneumophila in hospital water supplies.
Ten Legionella pneumophila strains isolated from different sources were analyzed according to their restriction fragment patterns obtained by cleavage of genomic DNA with NotI and SfiI and separation by pulsed field electrophoresis. Three L. pneumophila isolates from a nosocomial outbreak in Lübeck (Germany) and three other L. pneumophila strains independently isolated from a water tap located ...
متن کاملPersistence of Legionella in hospital water supplies and nosocomial Legionnaires' disease.
The molecular epidemiology of clinical and environmental Legionella species isolates was studied in seven hospitals from 1989 to 2006. The number of environmental pulsed field gel electrophoresis (PFGE) patterns ranged from one to nine according to the hospital. Genomic PFGE pattern persistence was observed in 71% of the hospitals, even after 17 years in some hospitals, and the relationship bet...
متن کاملDiscriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis.
Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.
متن کاملNosocomial Legionnaires' disease caused by Legionella pneumophila serogroup 5: laboratory and epidemiologic implications.
Environmental monitoring and clinical surveillance for Legionella species were done for 12 months as recommended by the Allegheny County Health Department (Pittsburgh). The water system of a hospital was found to be colonized with Legionella pneumophila serogroup 5. Three patients with nosocomial L. pneumophila serogroup 5 disease were subsequently diagnosed after laboratory tests for legionell...
متن کاملEnergy Conservation and the Promotion of Legionella pneumophila Growth: The Probable Role of Heat Exchangers in a Nosocomial Outbreak
OBJECTIVE To determine the source of a Legionella pneumophila serogroup 5 nosocomial outbreak and the role of the heat exchanger installed on the hot water system within the previous year. SETTING A 400-bed tertiary care university hospital in Sherbrooke, Canada. METHODS Hot water samples were collected and cultured for L. pneumophila from 25 taps (baths and sinks) within wing A and 9 taps in w...
متن کامل